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Test Subject Green has 30 levels, 10 more than its predecessor. Five are set in the Professor's lab at Xeno Industries, while 25 are set in Dr. Nastidious' Lab.
This section doesn't contain a video walkthrough. You can help the wiki by adding a video! To add a video, enter the Wikia editor, click the "Video" button, and follow its instructions. For a list of other pages that lack a video, please see Nitrome:Walkthroughs
The scientist notes that he seen shadows around the building and he feels like he is being watched. This level also introduces the enemy enzyme 'Monkey'.
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This section doesn't contain a video walkthrough. You can help the wiki by adding a video! To add a video, enter the Wikia editor, click the "Video" button, and follow its instructions. For a list of other pages that lack a video, please see Nitrome:Walkthroughs
This section is incomplete. You can help by adding the missing info.
In this level, the scientist is attacked. The scientists admits in the level's prologue that he must tighten security, but chooses to continue testing and tighten the security tomorrow. Alarms go off as the test starts, and the scientist is attacked.
This section doesn't contain a video walkthrough. You can help the wiki by adding a video! To add a video, enter the Wikia editor, click the "Video" button, and follow its instructions. For a list of other pages that lack a video, please see Nitrome:Walkthroughs
This section doesn't contain a video walkthrough. You can help the wiki by adding a video! To add a video, enter the Wikia editor, click the "Video" button, and follow its instructions. For a list of other pages that lack a video, please see Nitrome:Walkthroughs
This section doesn't contain a video walkthrough. You can help the wiki by adding a video! To add a video, enter the Wikia editor, click the "Video" button, and follow its instructions. For a list of other pages that lack a video, please see Nitrome:Walkthroughs
Monkey green enzymes - Green enzymes that have the ability to propel themselves up and move on the ceiling.
Mimic green enzymes - Green enzymes that change their appearance from a blob to something that looks like Blue. This transformation copies Blue's movements except for standing still. Also, when Blue fires, some time later the mimic green enzyme fires in the same place Blue did.
Nitrome has said that the game went into production right after Test Subject Blue. From the trailer released on April 20, 2011, Blue is the hero and green enzymes are the enemy. A sequel is currently on development, officially known as Test Subject Complete.
In level two, if Blue were to be killed by the monkey green enzyme, it would randomly start moving in between different areas between teleporters while not going through the teleporters at all. It would also become impossible fro the player to destroy the enzyme or the green enzyme destroying Blue. Later, a blog post reported that the glitch had been fixed.
On level eleven, if Blue died in a certain scenario, they could be trapped with no way out. This glitch was later reported fixed.
In level fifteen, if Blue jumps on a certain teleporter right above a laser, he will sometimes die, as if he landed on the laser instead of going through the teleporter to the one above it. (See image below.)
In level twenty, on occasion Blue reaches the end and collects the food (as meant to), gets sucked up the tube (also as meant), but the level does not end and the timer continues.
Sometimes, when Blue is killed and is sent back to his nearest checkpoint, he can do a super-jump by rapidly pressing jump and shoot at the same time while invincible, which may shoot Blue off of the screen, forcing the player to restart the level.
It is possible to get to the other side of a teleporter if right and left keys are pressed steadily and fast if Blue remains in the middle of a teleporter.
If the player holds down the shoot button and the up button, right as Blue is spawning, Blue will rocket upwards in the air. Also holding down the left or right keys can adjust flight direction.