The bats place three green gems, and then a red gem a little bit farther and a little bit higher then the green one. Then, the bats place four rock platforms underneath the green crystals, and the brown miner jumps onto the purple rock and runs to collect the three green gems while the rock platforms fall off-screen, revealing the word Nitrome.
The Nitrome letters are all floating in the air. The "N" and "E" of the Nitrome logo move over to each other, then turn around and shoot down all the other letters. The N and the E turn to face each other, with the N shooting the E and the E shooting the N, falling into place.
The letters N-I-T-R in green with frog's eyes, and the letters O-M-E in pink with bunny ears jump around before jumping into position, making the Nitrome logo. Every letter looks like a head on Ribbit.
The game cartridge for Super Treadmill is put into the Nitrome Enjoyment System. The "P" button is pressed several times. The cartridge is taken out, blowed on (only heard as a sound), and put back into the system. The P button is pressed on again, this time successful. The message shown when a game cartridge is put into the original Nitrome Enjoyment System is shown, with directions at the bottom saying to ignore it.
The Silly Sausage cartridge is about to be placed into the Nitrome Enjoyment System, but the Game Muscle cartridge is suddenly attached to the Silly Sausage cartridge. It is inserted into the Nitrome Enjoyment System and the "P" button is pressed. Notably, the Nitrome logo is placed on the top of the screen instead of the middle.
The game cartridge is inserted into the NES and the "P" button is pressed. The Nitrome jingle is heard, but abruptly loses pitch. Afterwards, the display of games in Mega Mash is seen, but there is a fizz on the screen, giving the impression of a "glitchy" game.
Next, the game cartridge is removed, and smacking noises are heard once it is out of the screen. It is then inserted back in the NES and "P" is pressed once more. The full Nitrome jingle is heard this time, with smoke trailing out of the game system.
Letters forming "The 100th Nitrome Game" are flying from the upper left corner. The background is pink with the Cuboy faces. The player hears a drum-roll, and suddenly a typical "Alarm-sound". The background is blinking red, and turns black/grey, Cuboy turning into a skull. Pistols shots come flying off screen, into the 'T' and 'E' of 'The' and the 'T' of '100th' disappears, along with the 'E' of 'Game', with the 'T' of 'Nitrome' moving a bit back. There are some bullet wounds in the letters. Then, some B.C. Bow Contest arrows fly into 'The 100th Nitrome Game'. Big rockets shoot in 'The 100th' and 'game', and explode, hitting the word 'Nitrome'. Little pieces of Nitrome are burning down. After that the typical Nitrome jingle is heard.
Similar to the first Cave Chaos, bats arrange a short platform of blue rocks that the brown miner crosses while collecting the gems above the platform. The game's background design and refined artwork are seen during this startup, however.
Several blocks, including a turret, move along a coloured line, which is followed by the Nitrome logo. When it reaches the peak, "N" stays there snoring, while "itrome" slides back down to the center. Then, "i" maneuvers under the "N" and shoots it, waking it up and completing the Nitrome logo.
The letters of "Nitrome", aside from the "r", move in a jerky motion towards the centre of the screen, either from the top or bottom. The letter "r" then starts to move towards the "t" position, but shifts to the right to fit in the missing hole.
Several vampire bats are seen hanging from the word "Nitrome", when suddenly several wooden stakes are fired from both side of the screen, killing the bats. One bat is left, which flies up above the word "Nitrome", only to be killed by a stake which is shot at it.
Many bubbles float upwards and an anglerfish appears with the Nitrome logo outside its mouth. Notably, the Nitrome jingle is sped up, much like the one in 8bit Doves. This is the last Flash game to use the Nitrome startup.
The word "Nitrome" is drawn on a blueprint paper in white pencil. After that, a scanner goes over the word sketch and then turns it into a 3-D image of the word. The word "demo" appears in small, flickering computer letters underneath.
A remake of the Feed Me startup, except having a green background instead of a brown background. A fly from the to-be-released sequel flies from the top of the screen in a slight wave motion and lands on the fly trap, only to become startled as the fly trap closes. The fly trap snaps shut to reveal the Nitrome logo, which then shines.
The letter "O" with a balloon attached appears floating above the almost complete word "Nitrome", when a hand appears and draws a circle that pops the balloon causing the O letter to fall and complete the Nitrome logo.
The letters "itrome" appears normal and then the stretchy dog (near the word) stretches to form the letter "N". Cuboy appears in a speech bubble at the end, the first appearance of what would become a regular occurrence throughout later Nitrome startups.
The word "Nitrome" is thrown from off screen (accompanied with a "Hiyah!" sound) and hits a wall, leaving cracks in the wall. The word then moves a bit back and falls on to the floor, bouncing a bit. Small thin pieces of the wall fall off (making a glass-like sound), exposing the brick part of the wall.
The word "Nitrome" appears upside-down, then the seagulls fly by and one stops and stands on the word, pushing the word downwards and causing it to rotate in a circle then appear normal. The startup does not use Cuboy at the end.
A mimic of the Turnament startup. The letters of "Nitrome", aside from the "o", and a slime appear stationary. The letters then move in a jerky motion towards the centre of the screen and then the letter "r" enters the screen and moves into position and hits the slime, which dies and reveals the "o". Again, the startup do not use the Cuboy speech bubble at the end.
A king holding a lever while on his throne watches two yellow shelled creatures bring the letters of the word Nitrome. The first shelled creature enters with the "O", "M", and "E" letters, drops them in that order, then leaves the screen and the second shelled creature enters with the "N", "I", "T", and "R" letters, dropping them in that order and completing the word "Nitrome". The second yellow shelled creature tries to walk off the screen, but as it walks over a panel on the ground the king pulls a lever which opens the panel and causes the shelled creature to fall into it.
The word "Nitrome" is green coloured, except for the N which is a puffing wall. A mouse moves up behind the N, which puffs out, pushing the rest of the word to the right and scaring the mouse so that it runs into its hole.
The screen is split diagonally into a yellow world at the top and blue world at the bottom. The yellow word Nitrome, standing on the diagonal line in the blue world and appearing inverted, jumps up, falls down and into the yellow world, where it lands on the ground and appears purple coloured and normal.
A wall with the word "nitrome" in pixel letters is seen, then the Flat Pack protagonist is thrown into the wall, and the word pops out in 3-D and falls to the ground. Cuboy in the speech bubble appears in 2D and then suddenly pops out in 3D.
An ancient temple floor is shown, upon which a BFC food container drops. After this, three chicken nuggets, fries, two chicken drumsticks, and coleslaw drop into the container. Blue sauce is applied horizontally across the food, as if squeezed out of a bottle. The letters of the word Nitrome then fall on to the food.
The letters of the word "Nitrome", appearing blue coloured and some having enemies, enter the screen moving in large circles towards the middle of the screen to form the word "nitrome". The word then changes to yellow, throwing all the enemies off the words.
The word "Nitrome" appears, except the "o" is replaced with a flag and has a hole on the ground in front of it. The sound of a ball being hit is heard and the ball moves quickly on to the screen, bounces off the wall, and is directed into the hole. As it enters the hole, the flag turns into a "o" and the hole disappears.